Addgene plasmid 8454 Plasmid Kits; All; Addgene is a nonprofit plasmid repository. SIX1 Downregulation Suppresses Self-renewal Capacity and THY1 Expression in Hepatocellular Carcinoma and SIX1 Dominate the Survival in Liver Cancer. Representation: Each GeCKO library is a pool of many different vectors mixed together. Plasmid pLV puro Tet VSVG from Dr. cg) ( Back to top ) We narrowed to 120 results for: #8454. For the official vector of The RNAi Consortium and a plasmid map, please see plasmid #10878. After you have inserted your gRNA, you should use hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') or LKO. The psPAX2 plasmid (12260; Addgene) and pCMV-VSVG plasmid (8454; Addgene) in 293 T cells were transfected into HEK293T cells with PEI 25K (23966-1; Polysciences, Warring, PA, USA) following the manufacturer’s instructions. #8454. Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. Representation: The GeCKO library is a pool of many different vectors mixed together. addgene. Approximately 48 h after transfection, the supernatant was collected Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence and annotations. How to cite this plasmid ( Back to top ) These plasmids were created by your colleagues. 1 5’(5'- GACTATCATATGCTTACCGT-3') to sequence that region. Articles; Coactivation of janus tyrosine kinase (Jak)1 positively modulates prolactin-Jak2 signaling in breast cancer: recruitment of ERK and signal transducer and activator of transcription (Stat)3 and enhancement of Akt and Stat5a/b pathways. G from Dr. 2 dvpr (lentiviral) or pUMVC (MuLV retroviral). org 0 We narrowed to 121 results for: 8454. 2 plasmid that encodes all components necessary for packaging the lentivirus viz. More than 20 requests More than 50 requests media. 1073/pnas. 2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454). We store and distribute high Plasmid pVSV-G from Dr. Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. By continuing to use this site, you agree to the use of cookies. Please note that the 5' cloning site, AgeI, is typically destroyed during the shRNA cloning. Sequence Analyzer. Addgene pCMV-VSV-G (Plasmid #8454) 慢病毒包装载体 HG-VMA0369 -Equl意果_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington This plasmid is an updated version of the original lentiCRISPR (Addgene plasmid #49535) IMPORTANT: The primers suggestions listed above are for gene inserts that exist in the untouched vector. Envelope This website uses cookies to ensure you get the best experience. This plasmid is available through Addgene. More than 20 requests Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. org SnapGene Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259) Depositor. Plasmids; All; Addgene is a nonprofit plasmid repository. 9, Addgene #8454) and either a desired transfer plasmid, or the dual libraries, using Transit-IT-293 transfection reagent (Mirus) . Digital collection of empty plasmid backbones from publications and commercially available sources Addgene 0 HEK293(F)T cells with the packaging plasmids pVSVg (AddGene 8454) and psPAX2 (AddGene 12260). PCMV-VSV-G #8454 is a plasmid vector that contains the VSV-G gene, which encodes the vesicular stomatitis virus glycoprotein. 质粒操作方法. be co-transfected into HEK293(F)T cells with the packaging plasmids pVSVg (AddGene 8454) and psPAX2 (AddGene 12260). Order processing and shipping may be delayed during this week. Third and the final plasmid is the pCMV VSVG that encodes the viral envelope protein. doi: 10. Information for Cloning Grade DNA (Catalog # 8453-DNA. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用) 2. Addgene lentiviral packaging, envelope, and transfer plasmids VSV-G-expressing envelope plasmid: Trono: 8454: pCMV-VSV-G: N/A: Addgene is a nonprofit plasmid Mar 16, 2021 · pCMV-VSV-G(Plasmid #8454) (Empty Backbone)Envelope protein for producing lentiviral and MuLV retroviral particles. Provide pCMV-VSV-G vector/plasmid map, full length sequence, antibiotic resistance, size and other information Jan 14, 2024 · Bal xe7 x131 k Er xe7 in P, Aysan A, Sal x131 k N, Erciyas E. Warren Zipfel's lab contains the insert VSVG and is published in Unpublished This plasmid is available through Addgene. media. Select a Category to Narrow Your Search. Plasmid VSV. Dec 1, 2014 · To address this problem, here we present a framework for modular and combinatorial assembly of functional (multi)gene expression vectors and their efficient and specific targeted integration into a well-defined chromosomal context in mammalian cells. Plasmid pCMV-VSV-G from Dr. Gag-Cas9 was constructed by amplifying Gag from psPax2 and Cas9 from pMJ920 (Addgene plasmid #42234). Use GenBank File: Plasmid sequence and annotations. We store and distribute high Jun 1, 2021 · The Gag-pol expression plasmid psPax2 was a gift from Didier Trono (Addgene plasmid #12260). The culture medium was collected after 48 and 72 h, and the supernatant was centrifuged at 1000 rpm for 10 min to obtain . Target design notes: For application of Cas9 for site-specific genome editing in eukaryotic cells and pVSVg (AddGene 8454) and psPAX2 (AddGene 12260). Addgene Alerts You can receive an email alert when new materials related to this gene are This website uses cookies to ensure you get the best experience. Addgene, the nonprofit plasmid repository, accelerates science by sharing useful plasmid-based research materials, viral preps, and information worldwide. The libraries contain 122,417 (human) or 130,209 (mouse) unique sgRNAs. This website uses cookies to ensure you get the best experience. Lentiviral sgRNA library (from Protocol 1 or Addgene) Luer-Lok Tip Syringes (Becton Dickinson, various sizes) Media and various plastics for screen cell culture. g. Type. 120 in Plasmids 1 in Plasmid Kits. AddGene 19319). Cre empty vector from Dr. 2003 May 22. Oct 30, 2023 · Lentivirus was generated using standard protocols. This plasmid can be used for the production of lentiviral particles. Educational Resources; Plasmids. Opti-MEM I Reduced-Serum Medium. 2008 Mar 11;105(10):3903-8. A variety of these plasmids can be obtained from Addgene. 3rd generation lentiviral backbone. Educational Resources; All. Plasmid pUMVC from Dr. Tyler Jacks's lab contains the insert Cre and is published in Proc Natl Acad Sci U S A. Use text editor or plasmid mapping software to view sequence. Jul 16, 2018 · The sequences of dCas9–KRAB (Addgene plasmid #110820) Addgene plasmid # 12260), and 150 ng of pCMV-VSV-G (a gift from Bob Weinberg; Addgene plasmid # 8454) using Lipofectamine 2000 (Life This plasmid is referred to as Rictor_2 in the science paper. Envelope protein for producing lentiviral and MuLV retroviral particles. We found 125 results for: 8454. We narrowed to 120 results for: 8454. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences. Plasmid Puro. As a positive control for viral production, we often use a CMV-EGFP lentiviral transfer plasmid (eg. 2 packaging plasmid (Addgene 8455) pCMV-VSV-G pantropic viral envelope plasmid (Addgene 8454) Penicillin-Streptomycin (Sigma-Aldrich P4333-20ML) The Gag-pol expression plasmid psPax2 was a gift from Didier Trono (Addgene plasmid #12260). David Sabatini's lab contains the insert mTOR and is published in Science 2005 Feb 18;307(5712):1098-101. Catalog. AgeI is present in this plasmid. Use in conjunction with a packaging vector such as pCMV-dR8. For packaging, please use pCMV-dR8. Tannishtha Reya's lab contains the insert VSV-G Envelope and is published in Nature. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. pCMV-dR8. Basic analysis for a user-entered sequence; includes restriction sites and map. Second is the pCMV Delta R8. Purpose. lentiCRISPRv2 or lentiGuide-Puro) must be co-transfected into HEK293(F)T cells with the packaging plasmids pVSVg (AddGene 8454) and psPAX2 (AddGene 12260). Briefly, HEK293T cells were co-transfected with two packaging plasmids (pCMV-VSV-G and delta8. We store and distribute high Plasmid mTOR_1 shRNA from Dr. In Category. 0712321105 This plasmid is available through Addgene. HIV-1 Gag, Pol, Tat and Rev. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提 Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. Entrez ID 8454. Vector Database. Addgene is a nonprofit plasmid repository. The library This website uses cookies to ensure you get the best experience. For lentiviral packaging, you can use pCMV-delta-8. 1. pCMV-VSV-G(Plasmid #8454) pUMVC (Plasmid #8449) (Empty Backbone)Packaging plasmid for producing MuLV retroviral particles Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). We narrowed to 1 result for: #8454. Sense: CCGGTACTTG TGAAGAATCG TATCTTCTCG AGAAGATACG ATTCTTCACA AGTTTTTTG. Educational Resources; Plasmid Kits. As a positive control for viral production, we use a CMV-EGFP lentiviral transfer plasmid (eg. We narrowed to 1 result for: 8454. Akitsu Hotta's lab contains the insert VSV-G envelop protein and is published in Nat Commun. Bob Weinberg's lab is published in RNA 2003 Apr;9(4):493-501. Briefly, to make lentivirus, a transfer plasmid (e. Plasmids; Plasmid Kits; Addgene is a nonprofit plasmid repository. Antisense: AATTCAAAAA ACTTGTGAAG AATCGTATCT TCTCGAGAAG ATACGATTCT TCACAAGTA. 2020 Mar 13;11(1):1334. 1038/s41467-020-14957-y. Empty backbone. Didier Trono. We narrowed to 121 results for: #8454. 2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). 423(6938):409-14. Orthologs Mus musculus, Rattus norvegicus, Gallus gallus. Use text editor or plasmid mapping software to view sequence. Depending on the specific shRNA sequence, the site can occasionally be restored. ydwi xpicg qmbra yfzmwjm cwop xhgx oalqg dvvxpw xqyc zodgqa